CDH4 Antibody
| Code | Size | Price |
|---|
| PSI-57-420-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Cadherin-4, Retinal cadherin, R-CAD, R-cadherin, CDH4
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
CDH4 is a classical cadherin from the cadherin superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization.
Background References:
- Miotto,E., Cancer Res. 64 (22), 8156-8159 (2004)
- Johnson,E., J. Biol. Chem. 279 (30), 31041-31049 (2004)
- Kitagawa,M., Biochem. Biophys. Res. Commun. 271 (2), 358-363 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This CDH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-203 amino acids from the N-terminal region of human CDH4.
NCBI Gene ID #:
1002
NCBI Official Name:
Cadherin-4
NCBI Official Symbol:
CDH4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
100 kDa
Protein Accession #:
P55283
Protein GI Number:
81175161
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
P55283
User NOte:
Optimal dilutions for each application to be determined by the researcher.