SQLE Antibody

ProSci
Product Code: PSI-56-547
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-547-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).
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SQLE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in A549 cell line lysates (35ug/lane).
SQLE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Squalene monooxygenase, Squalene epoxidase, SE, SQLE, ERG1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway.
Background References:
  • Bailey, S.D., et al. Diabetes Care (2010) In press :
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008)
  • Helms, M.W., et al. Br. J. Cancer 99(5):774-780(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SQLE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the N-terminal region of human SQLE.
NCBI Gene ID #:
6713
NCBI Official Name:
Squalene monooxygenase
NCBI Official Symbol:
SQLE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
64 kDa
Protein Accession #:
Q14534
Protein GI Number:
296439362
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity
Swissprot #:
Q14534
User NOte:
Optimal dilutions for each application to be determined by the researcher.