EGLN2 Antibody

ProSci
Product Code: PSI-63-600
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-600-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of EGLN2 Antibody in MDA-MB231, Hela cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human testis tissue reacted with EGLN2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of EGLN2 Antibody in MDA-MB231, Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with EGLN2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Egl nine homolog 2, Estrogen-induced tag 6, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 1, HIF-PH1, HIF-prolyl hydroxylase 1, HPH-1, Prolyl hydroxylase domain-containing protein 1, PHD1, EGLN2, EIT6
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
EGLN2 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.
Background References:
  • Semenza,G.L. et.al., Cell 107 (1), 1-3 (2001) Aprelikova,O., et.al., Cancer Res. 69 (2), 616-624 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EGLN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-221 amino acids from the Central region of human EGLN2.
NCBI Gene ID #:
112398
NCBI Official Name:
Egl nine homolog 2
NCBI Official Symbol:
EGLN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
Q96KS0
Protein GI Number:
32129513
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity
Swissprot #:
Q96KS0
User NOte:
Optimal dilutions for each application to be determined by the researcher.