TH Antibody

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ProSci
Product Code: PSI-62-556
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-556-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from SW480, U-87 MG cell line (from left to right), using TH Antibody at 1:1000 at each lane.
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Western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the detected the TH protein (arrow).
3 / 4
4 / 4
Flow cytometric analysis of K562 cells using TH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of lysates from SW480, U-87 MG cell line (from left to right), using TH Antibody at 1:1000 at each lane.
Western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the detected the TH protein (arrow).
Flow cytometric analysis of K562 cells using TH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, TH, TYH
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
TH is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons.
Background References:
  • Kuhn,D.M., et.al., J. Biol. Chem. 277 (16), 14336-14342 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 486-514 amino acids from the C-terminal region of human TH.
NCBI Gene ID #:
7054
NCBI Official Name:
Tyrosine 3-monooxygenase
NCBI Official Symbol:
TH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
P07101
Protein GI Number:
239938945
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Neuroscience
Swissprot #:
P07101
User NOte:
Optimal dilutions for each application to be determined by the researcher.