ESR2 Antibody

ProSci
Product Code: PSI-58-890
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-890-400ul400ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of HepG2 cells using ESR2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Flow cytometric analysis of HepG2 cells using ESR2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2, ESR2, ESTRB, NR3A2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
ESR2 is a nuclear hormone receptor. The protein binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner.
Background References:
  • Chen H., Lin R.J.Cell 90:569-580(1997)
  • Caira F., Antonson P.J. Biol. Chem. 275:5308-5317(2000)
  • Bu H., Kashireddy P.Biochem. Biophys. Res. Commun. 317:54-59(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Pig, Rat
Immunogen:
This ESR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-397 amino acids from the Central region of human ESR2.
NCBI Gene ID #:
2100
NCBI Official Name:
Estrogen receptor beta
NCBI Official Symbol:
ESR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
Q92731
Protein GI Number:
6166154
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction
Swissprot #:
Q92731
User NOte:
Optimal dilutions for each application to be determined by the researcher.