HMOX1 Antibody

ProSci
Product Code: PSI-55-728
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-728-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of HMOX1 using rabbit polyclonal HMOX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMOX1 gene.
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Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of HMOX1 using rabbit polyclonal HMOX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMOX1 gene.
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Heme oxygenase 1, HO-1, HMOX1, HO, HO1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
Background References:
  • Wu, M.M., et al. Toxicol. Appl. Pharmacol. 248(3):226-233(2010)
  • Bolisetty, S., et al. J. Am. Soc. Nephrol. 21(10):1702-1712(2010)
  • Bao, W., et al. PLoS ONE(8), E12371 (2010) :
  • Wu, M.M., et al. J. Biomed. Sci. 17, 70 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HMOX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-204 amino acids from the Central region of human HMOX1.
NCBI Gene ID #:
3162
NCBI Official Name:
Heme oxygenase 1
NCBI Official Symbol:
HMOX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
P09601
Protein GI Number:
123446
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P09601
User NOte:
Optimal dilutions for each application to be determined by the researcher.