VLDLR Antibody

Product Code: PSI-62-037

Supplier: ProSci

Code	Size	Price

PSI-62-037-400ul

400ul

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit Ig

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Fluorescence-activated cell sorting (FACS)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing THP-1 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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Antibody staining VLDLR in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

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Western Blot at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.

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Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.

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VLDLR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

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Documents

Further Information

Additional Names:

Very low-density lipoprotein receptor, VLDL receptor, VLDL-R, VLDLR

Application Note:

For FACS starting dilution is: 1:25

For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:2000

Background:

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene. [provided by RefSeq].

Background References:

Sakai, K., et al. Brain Res. 1276, 11-21 (2009)
Francis, P.J., et al. J. Med. Genet. 46(5):300-307(2009)
Ananyeva, N.M., et al. Blood Coagul. Fibrinolysis 19(6):543-555(2008)
Turkmen, S., et al. Eur. J. Hum. Genet. 16(9):1070-1074(2008)

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Rb

Immunogen:

This VLDLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-510 amino acids from the Central region of human VLDLR.

NCBI Gene ID #:

7436

NCBI Official Name:

Very low-density lipoprotein receptor

NCBI Official Symbol:

VLDLR

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

96 kDa

Protein Accession #:

P98155

Protein GI Number:

1730111

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Research Area:

Cancer,Obesity,Neuroscience,Signal Transduction

Swissprot #:

P98155

User NOte:

Optimal dilutions for each application to be determined by the researcher.