TSHB Antibody

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ProSci
Product Code: PSI-63-549
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-549-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Confocal immunofluorescent analysis of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western Blot at 1:1000 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Thyrotropin subunit beta, Thyroid-stimulating hormone subunit beta, TSH-B, TSH-beta, Thyrotropin beta chain, Thyrotropin alfa, TSHB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
TSHB is indispensable for the control of thyroid structure and metabolism.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TSHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of human TSHB.
NCBI Gene ID #:
7252
NCBI Official Name:
Thyrotropin subunit beta
NCBI Official Symbol:
TSHB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa
Protein Accession #:
P01222
Protein GI Number:
311033515
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P01222
User NOte:
Optimal dilutions for each application to be determined by the researcher.