PRKAA2 Antibody
| Code | Size | Price |
|---|
| PSI-55-462-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a catalytic subunit of
the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer
consisting of an alpha catalytic subunit, and non-catalytic beta
and gamma subunits. AMPK is an important energy-sensing enzyme that
monitors cellular energy status. In response to cellular metabolic
stresses, AMPK is activated, and thus phosphorylates and
inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy
beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in
regulating de novo biosynthesis of fatty acid and cholesterol.
Studies of the mouse counterpart suggest that this catalytic
subunit may control whole-body insulin sensitivity and is necessary
for maintaining myocardial energy homeostasis during ischemia.
Background References:
- Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)
- Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
- Jassim, G., et al. Pharmacopsychiatry (2010) In press :
- Bungard, D., et al. Science 329(5996):1201-1205(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat
Immunogen:
This PRKAA2 (Thr172) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the Central region of human PRKAA2 (Thr172).
NCBI Gene ID #:
5563
NCBI Official Name:
5'-AMP-activated protein kinase catalytic subunit alpha-2
NCBI Official Symbol:
PRKAA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
P54646
Protein GI Number:
20178276
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction,Autophagy
Swissprot #:
P54646
User NOte:
Optimal dilutions for each application to be determined by the researcher.