IsocitRe dehydrogenase Antibody
| Code | Size | Price |
|---|
| PSI-60-430-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, Isocitric dehydrogenase subunit alpha, NAD(+)-specific ICDH subunit alpha, IDH3A
Application Note:
For WB starting dilution is: 1:1000
Background:
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
Background References:
- Soundar, S., et al., J. Biol. Chem. 278(52):52146-52153 (2003).
- Weiss, C., et al., Biochemistry 39(7):1807-1816 (2000).
- Kim, Y.O., et al., J. Biol. Chem. 274(52):36866-36875 (1999).
- Huh, T.L., et al., Genomics 32(2):295-296 (1996).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Rat
Immunogen:
This Isocitrate dehydrogenase (IDH3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human Isocitrate dehydrogenase (IDH3).
NCBI Gene ID #:
3419
NCBI Official Name:
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
NCBI Official Symbol:
IDH3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
P50213
Protein GI Number:
1708399
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P50213
User NOte:
Optimal dilutions for each application to be determined by the researcher.