ADCY4 Antibody

ProSci
Product Code: PSI-64-161
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-161-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot in Hela cell line lysates (35ug/lane). ADCY4 (arrow) was detected using the purified Pab.
2 / 2
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot in Hela cell line lysates (35ug/lane). ADCY4 (arrow) was detected using the purified Pab.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Adenylate cyclase type 4, ATP pyrophosphate-lyase 4, Adenylate cyclase type IV, Adenylyl cyclase 4, ADCY4
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). Mouse studies show that adenylate cyclase 4, along with adenylate cyclases 2 and 3, is expressed in olfactory cilia, suggesting that several different adenylate cyclases may couple to olfactory receptors and that there may be multiple receptor-mediated mechanisms for the generation of cAMP signals.
Background References:
  • Rhim, J.H., et al. Aging Cell 5(6):451-461(2006)
  • Jiang, G., et al. Am. J. Physiol. Endocrinol. Metab. 284 (4), E671-E678 (2003)
  • Sunahara, R.K., et al. Mol. Interv. 2(3):168-184(2002)
  • Ludwig, M.G., et al. J. Recept. Signal Transduct. Res. 22 (1-4), 79-110 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ADCY4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-441 amino acids from the Central region of human ADCY4.
NCBI Gene ID #:
196883
NCBI Official Name:
Adenylate cyclase type 4
NCBI Official Symbol:
ADCY4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
120 kDa
Protein Accession #:
Q8NFM4
Protein GI Number:
25008336
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q8NFM4
User NOte:
Optimal dilutions for each application to be determined by the researcher.