ARNT Antibody
| Code | Size | Price |
|---|
| PSI-57-640-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, bHLHe2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta, HIF-1-beta, HIF1-beta, ARNT, BHLHE2
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For WB starting dilution is: 1:1000
Background:
The aryl hydrocarbon (Ah) receptor is involved in the
induction of several enzymes that participate in xenobiotic
metabolism. The ligand-free, cytosolic form of the Ah receptor is
complexed to heat shock protein 90. Binding of ligand, which
includes dioxin and polycyclic aromatic hydrocarbons, results in
translocation of the ligand-binding subunit only to the nucleus.
Induction of enzymes involved in xenobiotic metabolism occurs
through binding of the ligand-bound Ah receptor to xenobiotic
responsive elements in the promoters of genes for these enzymes.
This gene encodes a protein that forms a complex with the
ligand-bound Ah receptor, and is required for receptor function.
The encoded protein has also been identified as the beta subunit of
a heterodimeric transcription factor, hypoxia-inducible factor 1. A
t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion
protein, is associated with acute myeloblastic leukemia.
Alternative splicing results in multiple transcript variants.
Background References:
- Otsubo, K., et al. Cancer Genet. Cytogenet. 202(1):22-26(2010)
- Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
- Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
- Kewley, R.J., et al. Biochem. Biophys. Res. Commun. 338(1):660-667(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ARNT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-544 amino acids from the Central region of human ARNT.
NCBI Gene ID #:
405
NCBI Official Name:
Aryl hydrocarbon receptor nuclear translocator
NCBI Official Symbol:
ARNT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
87 kDa
Protein Accession #:
P27540
Protein GI Number:
114163
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P27540
User NOte:
Optimal dilutions for each application to be determined by the researcher.