SMAD9 Antibody

ProSci
Product Code: PSI-58-084
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-084-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western Blot at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Western blot analysis in A549 cell line lysates (35ug/lane).
3 / 3

Western Blot at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in A549 cell line lysates (35ug/lane).

Further Information

Additional Names:
Mothers against decapentaplegic homolog 9, MAD homolog 9, Mothers against DPP homolog 9, Madh6, SMAD family member 9, SMAD 9, Smad9, SMAD9, MADH6, MADH9
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Liu, Y., et al. FASEB J. 23(7):2299-2306(2009)
  • Su, D., et al. J. Biol. Chem. 284(18):12153-12164(2009)
  • Shintani, M., et al. J. Med. Genet. 46(5):331-337(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the Central region of human SMAD9.
NCBI Gene ID #:
4093
NCBI Official Name:
Mothers against decapentaplegic homolog 9
NCBI Official Symbol:
SMAD9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
O15198
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
O15198
User NOte:
Optimal dilutions for each application to be determined by the researcher.