SMAD4 Antibody
| Code | Size | Price |
|---|
| PSI-63-182-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Background:
SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.
Background References:
- Sekiya, T., et al., Biochem. Biophys. Res. Commun. 320(3):680-684 (2004).
- Horvath, L.G., et al., Prostate 59(3):234-242 (2004).
- Li, L., et al., Mol. Cell. Biol. 24(2):856-864 (2004).
- Wan, M., et al., J. Biol. Chem. 279(15):14484-14487 (2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from human SMAD4.
NCBI Gene ID #:
4089
NCBI Official Name:
Mothers against decapentaplegic homolog 4
NCBI Official Symbol:
SMAD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
Q13485
Protein GI Number:
13959561
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q13485
User NOte:
Optimal dilutions for each application to be determined by the researcher.