Protein Phosphatase 1 beta Antibody

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ProSci
Product Code: PSI-63-467
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-467-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent image of U251 cell stained with PPP1CB Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PPP1CB primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly.
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Western blot analysis of PPP1CB using rabbit polyclonal PPP1CB Antibody (K301) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene.
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Fluorescent image of U251 cell stained with PPP1CB Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PPP1CB primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Western blot analysis of PPP1CB using rabbit polyclonal PPP1CB Antibody (K301) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene.

Documents

Further Information

Additional Names:
Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, PPP1CB
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
Protein phosphatase-1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases involved in the dephosphorylation of a variety of proteins. These enzymes work in opposition to the protein kinases to control the level of phosphorylation. Protein phosphatase (PP1) is essential for cell division, and it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis, as well as in regulation of ionic conductances and long-term synaptic plasticity. PP1 has 3 catalytic subunits, designated alpha (PPP1CA), beta (PPP1CB), and gamma (PPP1CC).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus
Immunogen:
This Protein Phosphatase 1 beta (PPP1CB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human Protein Phosphatase 1 beta (PPP1CB).
NCBI Gene ID #:
5500
NCBI Official Name:
Serine/threonine-protein phosphatase PP1-beta catalytic subunit
NCBI Official Symbol:
PPP1CB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
P62140
Protein GI Number:
49065814
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P62140
User NOte:
Optimal dilutions for each application to be determined by the researcher.