COXI Antibody

ProSci
Product Code: PSI-64-065
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-065-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: mouse spleen lysate Lysates/proteins at 20 ug per lane.
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Confocal immunofluorescent analysis of COXI Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: mouse spleen lysate Lysates/proteins at 20 ug per lane.
Confocal immunofluorescent analysis of COXI Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Further Information

Additional Names:
Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I, MT-CO1, COI, COXI, MTCO1
Application Note:
For WB starting dilution is: 1:2000

For IF starting dilution is: 1:10~50
Background References:
  • Andrews, R.M., et al. Nat. Genet. 23 (2), 147 (1999) Anderson, S., et al. Nature 290(5806):457-465(1981)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Zebrafish, Mouse, Pig, Rabbit, Rat, Xenopus
Immunogen:
This COXI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids from the Central region of human COXI.
NCBI Gene ID #:
4512
NCBI Official Name:
Cytochrome c oxidase subunit 1
NCBI Official Symbol:
MT-CO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
P00395
Protein GI Number:
116977
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P00395
User NOte:
Optimal dilutions for each application to be determined by the researcher.