GPD2 Antibody

ProSci
Product Code: PSI-56-468
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-468-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
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GPD2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
GPD2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Glycerol-3-phosphate dehydrogenase, mitochondrial, GPD-M, GPDH-M, mtGPD, GPD2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.
Background References:
  • Barber, M.J., et al. PLoS ONE(3), E9763 (2010) :
  • Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009)
  • Daoud, H., et al. Hum. Genet. 124(6):649-658(2009)
  • Chowdhury, S.K., et al. Free Radic. Res. 41(10):1116-1124(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse
Immunogen:
This GPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 606-634 amino acids from the C-terminal region of human GPD2.
NCBI Gene ID #:
2820
NCBI Official Name:
Glycerol-3-phosphate dehydrogenase, mitochondrial
NCBI Official Symbol:
GPD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
81 kDa
Protein Accession #:
P43304
Protein GI Number:
229462943
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P43304
User NOte:
Optimal dilutions for each application to be determined by the researcher.