ECE1 Antibody

ProSci
Product Code: PSI-62-505
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-505-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of ECE1 Antibody in HepG2 cell line lysates (35ug/lane)
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ECE1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of ECE1 Antibody in HepG2 cell line lysates (35ug/lane)
ECE1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Endothelin-converting enzyme 1, ECE-1, ECE1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
ECE-1 is involved in proteolytic processing of endothelin precursors to biologically active peptides.
Background References:
  • Cottrell,G.S., et.al., J. Biol. Chem. 284 (33), 22411-22425 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This ECE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the Central region of human ECE1.
NCBI Gene ID #:
1889
NCBI Official Name:
Endothelin-converting enzyme 1
NCBI Official Symbol:
ECE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
87 kDa
Protein Accession #:
P42892
Protein GI Number:
1706563
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P42892
User NOte:
Optimal dilutions for each application to be determined by the researcher.