ERG25 Antibody

ProSci
Product Code: PSI-57-377
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-377-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in 293 cell line lysates (35ug/lane).
2 / 2
ERG25 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tisssue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in 293 cell line lysates (35ug/lane).
ERG25 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tisssue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Methylsterol monooxygenase 1, C-4 methylsterol oxidase, MSMO1, DESP4, ERG25, SC4MOL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Sterol-C4-mehtyl oxidase-like protein was isolated based on its similarity to the yeast ERG25 protein. It contains a set of putative metal binding motifs with similarity to that seen in a family of membrane desaturases-hydroxylases. The protein is localized to the endoplasmic reticulum membrane and is believed to function in cholesterol biosynthesis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Background References:
  • Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008)
  • Lim, J., et al. Cell 125(4):801-814(2006)
  • Li, L., et al. J. Biol. Chem. 271(28):16927-16933(1996)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ERG25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the C-terminal region of human ERG25.
NCBI Gene ID #:
6307
NCBI Official Name:
Methylsterol monooxygenase 1
NCBI Official Symbol:
MSMO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
Q15800
Protein GI Number:
2498340
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q15800
User NOte:
Optimal dilutions for each application to be determined by the researcher.