NDUFAB1 Antibody

ProSci
Product Code: PSI-57-505
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-505-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
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NDUFAB1 immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDUFAB1 for immunohistochemistry.

Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
NDUFAB1 immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDUFAB1 for immunohistochemistry.

Further Information

Additional Names:
Acyl carrier protein, mitochondrial, ACP, CI-SDAP, NADH-ubiquinone oxidoreductase 96 kDa subunit, NDUFAB1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Carrier of the growing fatty acid chain in fatty acid biosynthesis in mitochondria. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain (By similarity).
Background References:
  • Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
  • Feng, D., et al. J. Biol. Chem. 284(17):11436-11445(2009)
  • Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008)
  • Zhang, X., et al. BMC Cell Biol. 9,(2008) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This NDUFAB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-156 amino acids from the C-terminal region of human NDUFAB1.
NCBI Gene ID #:
4706
NCBI Official Name:
Acyl carrier protein, mitochondrial
NCBI Official Symbol:
NDUFAB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
O14561
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
O14561
User NOte:
Optimal dilutions for each application to be determined by the researcher.