ENOB Antibody

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ProSci
Product Code: PSI-61-577
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-577-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in RD cell line and mouse skeletal muscle,rat skeletal muscle lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis in RD cell line and mouse skeletal muscle,rat skeletal muscle lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, MSE, Skeletal muscle enolase, ENO3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ENOB is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.
Background References:
  • Aurino,S., Acta Myol 27, 90-97 (2008)
  • Giallongo,A., Eur. J. Biochem. 214 (2), 367-374 (1993)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.
NCBI Gene ID #:
2027
NCBI Official Name:
Beta-enolase
NCBI Official Symbol:
ENO3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
P13929
Protein GI Number:
425906077
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P13929
User NOte:
Optimal dilutions for each application to be determined by the researcher.