ECE-1 Antibody

ProSci
Product Code: PSI-62-503
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-503-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis of ECE-1 Antibody in A2058 cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human skin tissue reacted with ECE-1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis of ECE-1 Antibody in A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skin tissue reacted with ECE-1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Endothelin-converting enzyme 1, ECE-1, ECE1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ECE-1 is involved in proteolytic processing of endothelin precursors to biologically active peptides.
Background References:
  • Cottrell,G.S., et.al., J. Biol. Chem. 284 (33), 22411-22425 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This ECE-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 528-556 amino acids from the C-terminal region of human ECE-1.
NCBI Gene ID #:
1889
NCBI Official Name:
Endothelin-converting enzyme 1
NCBI Official Symbol:
ECE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
87 kDa
Protein Accession #:
P42892
Protein GI Number:
1706563
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P42892
User NOte:
Optimal dilutions for each application to be determined by the researcher.