MDH1 Antibody

ProSci
Product Code: PSI-61-590
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-590-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis in HL-60,THP-1 cell line lysates (35ug/lane).
2 / 3
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis in HL-60,THP-1 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase, Diiodophenylpyruvate reductase, MDH1, MDHA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
Background References:
  • Lee,S.M., et.al., Cell Death Differ. 16 (5), 738-748 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Pig, Rat
Immunogen:
This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1.
NCBI Gene ID #:
4190
NCBI Official Name:
Malate dehydrogenase, cytoplasmic
NCBI Official Symbol:
MDH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
P40925
Protein GI Number:
1708967
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P40925
User NOte:
Optimal dilutions for each application to be determined by the researcher.