PSMAL Antibody

ProSci
Product Code: PSI-61-778
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-778-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Immunohistochemical analysis of paraffin-embedded H. liver section using PSMAL Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
2 / 5
Western blot analysis of lysate from human kidney tissue lysate, using PSMAL Antibody at 1:1000.
3 / 5
4 / 5
PSMAL Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
5 / 5
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded H. liver section using PSMAL Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysate from human kidney tissue lysate, using PSMAL Antibody at 1:1000.
PSMAL Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Putative N-acetylated-alpha-linked acidic dipeptidase, NAALADase, 34--, Cell growth-inhibiting gene 26 protein, Prostate-specific membrane antigen-like protein, Putative folate hydrolase 1B, FOLH1B, PSMAL
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
PSMAL has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity (By similarity). PSMAL exhibits a dipeptidyl-peptidase IV type activity
Background References:
  • O'Keefe, D.S., et al. Prostate 58(2):200-210(2004)
  • O'Keefe, D.S., et al. Biochim. Biophys. Acta 1443 (1-2), 113-127 (1998)
  • Maraj, B.H., et al. Cytogenet. Cell Genet. 81(1):3-9(1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PSMAL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-183 amino acids from the Central region of human PSMAL.
NCBI Gene ID #:
219595
NCBI Official Name:
Putative N-acetylated-alpha-linked acidic dipeptidase
NCBI Official Symbol:
FOLH1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q9HBA9
Protein GI Number:
74718738
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q9HBA9
User NOte:
Optimal dilutions for each application to be determined by the researcher.