Aldolase Antibody

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ProSci
Product Code: PSI-61-456
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-456-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from 293,RD,mouse NIH/3T3,rat L6 cell line (from left to right),using ALDOA Antibody at 1:1000 at each lane.
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of lysates from 293,RD,mouse NIH/3T3,rat L6 cell line (from left to right),using ALDOA Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA, ALDA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.
Background References:
  • Gizak,A., Proteins 72 (1), 209-216 (2008)
  • Lu,J., Biochem. Biophys. Res. Commun. 369 (3), 948-952 (2008)
  • Valis,K., Mol. Cell. Biochem. 311 (1-2), 225-231 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rabbit, Rat
Immunogen:
This Aldolase (ALDOA) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human Aldolase (ALDOA).
NCBI Gene ID #:
226
NCBI Official Name:
Fructose-bisphosphate aldolase A
NCBI Official Symbol:
ALDOA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
P04075
Protein GI Number:
113606
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P04075
User NOte:
Optimal dilutions for each application to be determined by the researcher.