ALOX15 Antibody

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ProSci
Product Code: PSI-63-276
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-276-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in Hela(lane 1),A549(lane 2),K562(lane 3),MDA-MB435(lane 4) cell line lysates (35ug/lane).
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Confocal immunofluorescent analysis of ALOX15 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela(lane 1),A549(lane 2),K562(lane 3),MDA-MB435(lane 4) cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of ALOX15 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Arachidonate 15-lipoxygenase, 15-LOX, 15-LOX-1, 12/15-lipoxygenase, Arachidonate 12-lipoxygenase, leukocyte-type, 12-LOX, Arachidonate omega-6 lipoxygenase, ALOX15, LOG15
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.
Background References:
  • Tang,Y., Int. J. Cancer 124 (7), 1545-1551 (2009)
  • Bevan,S., Stroke 40 (3), 696-701 (2009)
  • Zuo,X., J. Biol. Chem. 283 (46), 31341-31347 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ALOX15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-643 amino acids from the C-terminal region of human ALOX15.
NCBI Gene ID #:
246
NCBI Official Name:
Arachidonate 15-lipoxygenase
NCBI Official Symbol:
ALOX15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
P16050
Protein GI Number:
126396
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P16050
User NOte:
Optimal dilutions for each application to be determined by the researcher.