PML Sumoylation Site Antibody
| Code | Size | Price |
|---|
| PSI-61-401-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Protein PML, Promyelocytic leukemia protein, RING finger protein 71, Tripartite motif-containing protein 19, PML, MYL, PP8675, RNF71, TRIM19
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL).
Background References:
- Puccetti, E., et al., Oncogene 22(44):6900-6908 (2003).
- Favre, M., et al., J. Acquir. Immune Defic. Syndr. 34(2):127-133 (2003).
- Kawai, T., et al., Mol. Cell. Biol. 23(17):6174-6186 (2003).
- Louria-Hayon, I., et al., J. Biol. Chem. 278(35):33134-33141 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PML Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-499 amino acids from human PML Sumoylation Site.
NCBI Gene ID #:
5371
NCBI Official Name:
Protein PML
NCBI Official Symbol:
PML
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
98 kDa
Protein Accession #:
P29590
Protein GI Number:
215274219
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P29590
User NOte:
Optimal dilutions for each application to be determined by the researcher.