EDNRA Antibody

ProSci
Product Code: PSI-62-288
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-288-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of EDNRA antibody in CEM cell line lysates (35ug/lane)
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Confocal immunofluorescent analysis of EDNRA Antibody with HepG2 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of EDNRA antibody in CEM cell line lysates (35ug/lane)
Confocal immunofluorescent analysis of EDNRA Antibody with HepG2 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Further Information

Additional Names:
Endothelin-1 receptor, Endothelin A receptor, ET-A, ETA-R, hET-AR, EDNRA, ETA, ETRA
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
EDNRA is a receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Background References:
  • Cardillo,C., Arthritis Rheum. 60 (6), 1840-1844 (2009) Hayzer,D.J., Am. J. Med. Sci. 304 (4), 231-238 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This EDNRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human EDNRA.
NCBI Gene ID #:
1909
NCBI Official Name:
Endothelin-1 receptor
NCBI Official Symbol:
EDNRA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
P25101
Protein GI Number:
119606
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction,Growth Factor
Swissprot #:
P25101
User NOte:
Optimal dilutions for each application to be determined by the researcher.