GNAS Antibody
| Code | Size | Price |
|---|
| PSI-59-966-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS (HGNC:4392)
Application Note:
For WB starting dilution is: 1:1000
Background:
This locus has a highly complex imprinted expression
pattern. It gives rise to maternally, paternally, and biallelically
expressed transcripts that are derived from four alternative
promoters and 5' exons. Some transcripts contains a differentially
methylated region (DMR) at their 5' exons, and this DMR is commonly
found in imprinted genes and correlates with transcript expression.
An antisense transcript is produced from an overlapping locus on
the opposite strand. One of the transcripts produced from this
locus, and the antisense transcript, are paternally expressed
noncoding RNAs, and may regulate imprinting in this region. In
addition, one of the transcripts contains a second overlapping ORF,
which encodes a structurally unrelated protein - Alex. Alternative
splicing of downstream exons is also observed, which results in
different forms of the stimulatory G-protein alpha subunit, a key
element of the classical signal transduction pathway linking
receptor-ligand interactions with the activation of adenylyl
cyclase and a variety of cellular reponses. Multiple transcript
variants encoding different isoforms have been found for this gene.
Mutations in this gene result in pseudohypoparathyroidism type 1a,
pseudohypoparathyroidism type 1b, Albright hereditary
osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright
syndrome, progressive osseus heteroplasia, polyostotic fibrous
dysplasia of bone, and some pituitary tumors.
Background References:
- Idziaszczyk, S., et al. Cancer Genet. Cytogenet. 202(1):67-69(2010)
- Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
- Tominaga, E., et al. Gynecol. Oncol. 118(2):160-166(2010)
- Park, C.H., et al. Ann. Clin. Lab. Sci. 40(3):261-266(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GNAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-244 amino acids from the C-terminal region of human GNAS.
NCBI Gene ID #:
2778
NCBI Official Name:
Neuroendocrine secretory protein 55
NCBI Official Symbol:
GNAS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
O95467
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
O95467
User NOte:
Optimal dilutions for each application to be determined by the researcher.