HSPA1A/HSPA1B Antibody

ProSci
Product Code: PSI-63-005
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-005-400ul400ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of HSPA1A using rabbit polyclonal HSPA1A Antibody (S41) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2).

Western blot analysis of HSPA1A using rabbit polyclonal HSPA1A Antibody (S41) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2).

Further Information

Additional Names:
Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70
Application Note:
For WB starting dilution is: 1:1000
Background:
HSPA1A is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.
Background References:
  • Mueller, T., et al., Transplantation 78(2):292-295 (2004).
  • Fekete, A., et al., Pediatr. Res. 54(4):452-455 (2003).
  • Broquet, A.H., et al., J. Biol. Chem. 278(24):21601-21606 (2003).
  • Bruce, C.R., et al., Diabetes 52(9):2338-2345 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Monkey, Rat, Chicken, Xenopus, Yeast, C. Elegans, Hamster, Zebrafish, Drosophila
Immunogen:
This HSPA1A/HSPA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-48 amino acids from human HSPA1A/HSPA1B.
NCBI Gene ID #:
3303, 3304
NCBI Official Name:
Heat shock 70 kDa protein 1A/1B
NCBI Official Symbol:
HSPA1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70 kDa
Protein Accession #:
P08107
Protein GI Number:
147744565
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P08107
User NOte:
Optimal dilutions for each application to be determined by the researcher.