VE Cadherin Antibody

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ProSci
Product Code: PSI-61-455
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-455-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 2
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
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Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD144, CDH5
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
CDH5 is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.
Background References:
  • Breviario F.,Arterioscler. Thromb. Vasc. Biol. 15:1229-1239(1995)
  • Ali J.,Microcirculation 4:267-277(1997)
  • Suzuki S.,Cell Regul. 2:261-270(1991)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This VE Cadherin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-134 amino acids from the N-terminal region of human VE Cadherin.
NCBI Gene ID #:
1003
NCBI Official Name:
Cadherin-5
NCBI Official Symbol:
CDH5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
88 kDa
Protein Accession #:
P33151
Protein GI Number:
322510142
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P33151
User NOte:
Optimal dilutions for each application to be determined by the researcher.