MGAT5 Antibody

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ProSci
Product Code: PSI-57-289
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-289-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A, Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase, GlcNAc-T V, GNT-V, Mannoside acetylglucosaminyltransferase 5, N-acetylglucosaminyl-transferase V, MGAT5, GGNT5
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, a glycosyltransferase involved in the synthesis of protein-bound and lipid-bound oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the encoded protein's activity may correlate with the progression of invasive malignancies.
Background References:
  • Dick, D.M., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010) :
  • Brynedal, B., et al. J. Neuroimmunol. 220 (1-2), 120-124 (2010) :
  • Benson, V., et al. Int. Immunol. 22(3):167-177(2010)
  • Wang, C., et al. J. Cell. Biochem. 109(1):113-123(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MGAT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 652-680 amino acids from the C-terminal region of human MGAT5.
NCBI Gene ID #:
4249
NCBI Official Name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
NCBI Official Symbol:
MGAT5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
Q09328
Protein GI Number:
1169980
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q09328
User NOte:
Optimal dilutions for each application to be determined by the researcher.