TIEG Antibody

ProSci
Product Code: PSI-55-702
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-702-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of TIEG using rabbit polyclonal TIEG Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TIEG gene.
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Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of TIEG using rabbit polyclonal TIEG Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TIEG gene.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Krueppel-like factor 10, EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1, TGFB-inducible early growth response protein 1, TIEG-1, KLF10, TIEG, TIEG1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Transcriptional repressor involved in the regulation of cell growth. Inhibits cell growth. Binds to the consensus sequence 5'-GGTGTG-3'.
Background References:
  • Kim, J., et al. Biochem. Biophys. Res. Commun. 401(3):412-416(2010)
  • Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
  • Subramaniam, M., et al. Biofactors 36(1):8-18(2010)
  • Johnatty, S.E., et al. PLoS Genet.(7), E1001016 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TIEG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-225 amino acids from the Central region of human TIEG.
NCBI Gene ID #:
7071
NCBI Official Name:
Krueppel-like factor 10
NCBI Official Symbol:
KLF10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
Q13118
Protein GI Number:
11387050
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q13118
User NOte:
Optimal dilutions for each application to be determined by the researcher.