PPAP2C Antibody

ProSci
Product Code: PSI-61-512
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-512-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-PPAP2C Antibody in NCI-H460 cell line lysates (35ug/lane).
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Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of anti-PPAP2C Antibody in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Lipid phosphate phosphohydrolase 2, PAP2-gamma, PAP2-G, Phosphatidate phosphohydrolase type 2c, Phosphatidic acid phosphatase 2c, PAP-2c, PAP2c, PPAP2C, LPP2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
PPAP2C is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is similar to phosphatidic acid phosphatase type 2A (PPAP2A) and type 2B (PPAP2B). All three proteins contain 6 transmembrane regions, and a consensus N-glycosylation site. This protein has been shown to possess membrane associated PAP activity.
Background References:
  • Long,J.S., Biochem. J. 411 (2), 371-377 (2008)
  • Morris,K.E., J. Biol. Chem. 281 (14), 9297-9306 (2006)
  • Nanjundan,M., Am. J. Physiol. Lung Cell Mol. Physiol. 284 (1), L1-L23 (2003)
  • Zhang,N., Genesis 27 (4), 137-140 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This PPAP2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-254 amino acids from the C-terminal region of human PPAP2C.
NCBI Gene ID #:
8612
NCBI Official Name:
Lipid phosphate phosphohydrolase 2
NCBI Official Symbol:
PPAP2C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
O43688
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
O43688
User NOte:
Optimal dilutions for each application to be determined by the researcher.