EGFR Antibody

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ProSci
Product Code: PSI-60-735
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-735-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. skin section using EGFR Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Western blot analysis of lysates from A431, HUVEC cell line (from left to right), using EGFR Antibody at 1:1000 at each lane.
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Immunohistochemical analysis of paraffin-embedded H. skin section using EGFR Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysates from A431, HUVEC cell line (from left to right), using EGFR Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
Background References:
  • Abdallah, R.T., et al. J. Biol. Chem. 285(45):35206-35215(2010)
  • Lu, C., et al. Mol. Cell. Biol. 30(22):5432-5443(2010)
  • Rosell, R., et al. Ann. N. Y. Acad. Sci. 1210, 45-52 (2010) :
  • Hata, A., et al.Thorac Oncol 5(10):1524-1528(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Chicken
Immunogen:
This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the N-terminal region of human EGFR.
NCBI Gene ID #:
1956
NCBI Official Name:
Epidermal growth factor receptor
NCBI Official Symbol:
EGFR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
134 kDa
Protein Accession #:
P00533
Protein GI Number:
2811086
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P00533
User NOte:
Optimal dilutions for each application to be determined by the researcher.