HSPCB Antibody

ProSci
Product Code: PSI-63-776
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-776-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Western blot analysis in A431,H-4-II-E,Hela,rat L6 cell line lysates (35ug/lane).
3 / 3

Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in A431,H-4-II-E,Hela,rat L6 cell line lysates (35ug/lane).

Further Information

Additional Names:
Heat shock protein HSP 90-beta, HSP 90, Heat shock 84 kDa, HSP 84, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
HSPCB proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSPCB proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress.
Background References:
  • Chadli,A., et.al., J. Biol. Chem. 285 (6), 4224-4231 (2010)
  • Zhang,T., et.al., J. Biol. Chem. 284 (51), 35381-35389 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Rat
Immunogen:
This HSPCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-74 amino acids from the N-terminal region of human HSPCB.
NCBI Gene ID #:
3326
NCBI Official Name:
Heat shock protein HSP 90-beta
NCBI Official Symbol:
HSP90AB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
83 kDa
Protein Accession #:
P08238
Protein GI Number:
17865718
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
P08238
User NOte:
Optimal dilutions for each application to be determined by the researcher.