WISP2 Antibody
| Code | Size | Price |
|---|
| PSI-62-200-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
WNT1-inducible-signaling pathway protein 2, WISP-2, CCN family member 5, Connective tissue growth factor-like protein, CTGF-L, Connective tissue growth factor-related protein 58, WISP2, CCN5, CT58, CTGFL
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
Wisp2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. Wisp2 lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.
Background References:
- Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). Banerjee, S., et al., Neoplasia 5(1):63-73 (2003). Kumar, S., et al., J. Biol. Chem. 274(24):17123-17131 (1999). Pennica, D., et al., Proc. Natl. Acad. Sci. U.S.A. 95(25):14717-14722 (1998). Saxena, N., et al., Mol. Cell. Biochem. 228 (1-2), 99-104 (2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This WISP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-116 amino acids from the Central region of human WISP2.
NCBI Gene ID #:
8839
NCBI Official Name:
WNT1-inducible-signaling pathway protein 2
NCBI Official Symbol:
WISP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
O76076
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
O76076
User NOte:
Optimal dilutions for each application to be determined by the researcher.