PKC epsilon Antibody
| Code | Size | Price |
|---|
| PSI-62-593-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Protein kinase C epsilon type, nPKC-epsilon, PRKCE, PKCE
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC epsilon is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.
Background References:
- Gray, M.O., et al., J. Biol. Chem. 279(5):3596-3604 (2004).
- Ling, M., et al., Exp. Cell Res. 292(1):135-150 (2004).
- McJilton, M.A., et al., Oncogene 22(39):7958-7968 (2003).
- Robu, V.G., et al., J. Biol. Chem. 278(48):48154-48161 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PKC epsilon antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the N-terminal region of human PKC epsilon.
NCBI Gene ID #:
5581
NCBI Official Name:
Protein kinase C epsilon type
NCBI Official Symbol:
PRKCE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
84 kDa
Protein Accession #:
Q02156
Protein GI Number:
400135
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q02156
User NOte:
Optimal dilutions for each application to be determined by the researcher.