TIEG Antibody

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ProSci
Product Code: PSI-63-180
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-180-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: 293T whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: human kidney lysates Lane 4: human lung lysates Lysates/proteins at 20 ug per lane.
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Western blot analysis of anti-TIEG Antibody pre-incubated with and without blocking peptide in CEM cell line lysate.
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Western Blot at 1:2000 dilution Lane 1: 293T whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: human kidney lysates Lane 4: human lung lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-TIEG Antibody pre-incubated with and without blocking peptide in CEM cell line lysate.

Documents

Further Information

Additional Names:
Krueppel-like factor 10, EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1, TGFB-inducible early growth response protein 1, TIEG-1, KLF10, TIEG, TIEG1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
TIEG is a transcriptional repressor involved in the regulation of cell growth. TIEG binds to the consensus sequence 5'-GGTGTG-3'and inhibits cell growth.
Background References:
  • Hawse,J.R., Mol. Endocrinol. 22 (7), 1579-1595 (2008)
  • Ivanov,S.V., Biochem. Biophys. Res. Commun. 370 (4), 536-540 (2008)
  • Jin,W., FEBS Lett. 581 (20), 3826-3832 (2007)
  • Fueki,N., Respiration 74 (4), 454-459 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TIEG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human TIEG.
NCBI Gene ID #:
7071
NCBI Official Name:
Krueppel-like factor 10
NCBI Official Symbol:
KLF10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
Q13118
Protein GI Number:
11387050
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q13118
User NOte:
Optimal dilutions for each application to be determined by the researcher.