RPH3AL Antibody

ProSci
Product Code: PSI-55-383
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-383-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western Blot at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 2
RPH3AL Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western Blot at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.
RPH3AL Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Rab effector Noc2, No C2 domains protein, Rabphilin-3A-like protein, RPH3AL, NOC2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In pressShanmugam, C., et al. Biotech Histochem 84(2):39-45(2009) Katkoori, V.R., et al. Front. Biosci. 13, 1050-1061 (2008)Matsumoto, M., et al. Proc. Natl. Acad. Sci. U.S.A. 101(22):8313-8318(2004) Manabe, S., et al. Biochem. Biophys. Res. Commun. 316(1):218-225(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RPH3AL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-237 amino acids from the C-terminal region of human RPH3AL.
NCBI Gene ID #:
9501
NCBI Official Name:
Rab effector Noc2
NCBI Official Symbol:
RPH3AL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
Q9UNE2
Protein GI Number:
74735140
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q9UNE2
User NOte:
Optimal dilutions for each application to be determined by the researcher.