MFAP5 Antibody

ProSci
Product Code: PSI-62-819
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-819-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of MFAP5 antibody in K562 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MFAP5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of MFAP5 using rabbit polyclonal MFAP5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.

Western blot analysis of MFAP5 antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MFAP5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of MFAP5 using rabbit polyclonal MFAP5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.

Further Information

Additional Names:
Microfibrillar-associated protein 5, MFAP-5, MP25, Microfibril-associated glycoprotein 2, MAGP-2, MFAP5, MAGP2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.
Background References:
  • Albig,A.R., Becenti,D.J. Microvasc. Res. 76 (1), 7-14 (2008)
  • Miyamoto,A., Lau,R. J. Biol. Chem. 281 (15), 10089-10097 (2006)
  • Penner,A.S., Rock,M.J. J. Biol. Chem. 277 (38), 35044-35049 (2002)
  • Hatzinikolas,G. J. Biol. Chem. 273 (45), 29309-29314 (1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This MFAP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human MFAP5.
NCBI Gene ID #:
8076
NCBI Official Name:
Microfibrillar-associated protein 5
NCBI Official Symbol:
MFAP5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
Q13361
Protein GI Number:
2498553
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q13361
User NOte:
Optimal dilutions for each application to be determined by the researcher.