EREG Antibody

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ProSci
Product Code: PSI-61-668
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-668-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing HepG2 cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Antibody staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: A549 whole cell lysates Lane 3: NCI-H1299 whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + A549 whole cell lysates Lysates/proteins at 20 ug per lane.
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Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysates Lane 2: human placenta lysates Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
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Overlay histogram showing HepG2 cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Antibody staining EREG in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: A549 whole cell lysates Lane 3: NCI-H1299 whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysates Lane 2: human placenta lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Proepiregulin, Epiregulin, EPR, EREG
Application Note:
For FACS starting dilution is: 1:25

For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
EREG is a member of the epidermal growth factor family. EREG can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors.
Background References:
  • Ben-Ami, I., et al. Hum. Reprod. 24(1):176-184(2009)
  • Cho, M.C., et al. Biochem. Biophys. Res. Commun. 377(3):832-837(2008)
  • Lasky-Su, J., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EREG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the C-terminal region of human EREG.
NCBI Gene ID #:
2069
NCBI Official Name:
Proepiregulin
NCBI Official Symbol:
EREG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
O14944
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
O14944
User NOte:
Optimal dilutions for each application to be determined by the researcher.