GCDFP-15 Antibody

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ProSci
Product Code: PSI-55-647
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-647-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
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Confocal immunofluorescent analysis of GCDFP-15 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of GCDFP-15 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Prolactin-inducible protein, Gross cystic disease fluid protein 15, GCDFP-15, Prolactin-induced protein, Secretory actin-binding protein, SABP, gp17, PIP, GCDFP15, GPIP4
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
Background References:
  • Bryborn, M., et al. Allergy 65(2):220-228(2010)
  • Wang, L.J., et al. Appl. Immunohistochem. Mol. Morphol. 17(6):505-511(2009)
  • Yan, Z., et al. Diagn. Cytopathol. 37(7):475-478(2009)
  • Debily, M.A., et al. PLoS ONE(3), E4696 (2009) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GCDFP-15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the C-terminal region of human GCDFP-15.
NCBI Gene ID #:
5304
NCBI Official Name:
Prolactin-inducible protein
NCBI Official Symbol:
PIP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
P12273
Protein GI Number:
134170
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P12273
User NOte:
Optimal dilutions for each application to be determined by the researcher.