SRC Antibody

ProSci
Product Code: PSI-63-539
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-539-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from A375 cell line, using SRC Antibody (Y419) at 1:1000.
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Western blot analysis of SRC Antibody (Y419) in HepG2 cell line lysates (35ug/lane)
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Confocal immunofluorescent analysis of SRC Antibody (Y419) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Western blot analysis of lysate from A375 cell line, using SRC Antibody (Y419) at 1:1000.
Western blot analysis of SRC Antibody (Y419) in HepG2 cell line lysates (35ug/lane)
Confocal immunofluorescent analysis of SRC Antibody (Y419) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Further Information

Additional Names:
Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src, SRC, SRC1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
SRC is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.
Background References:
  • Muller,A.J., et.al., Mol. Cell. Biol. 12 (11), 5087-5093 (1992)
  • MacAuley,A., et.al., Oncogene(1), 117-124 (1993)
  • den Hertog,J.,et.al., EMBO J. 12 (10), 3789-3798 (1993)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Zebrafish, Pig, Chicken, Xenopus, B
Immunogen:
This SRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-424 amino acids from human SRC.
NCBI Gene ID #:
6714
NCBI Official Name:
Proto-oncogene tyrosine-protein kinase Src
NCBI Official Symbol:
SRC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
P12931
Protein GI Number:
125711
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P12931
User NOte:
Optimal dilutions for each application to be determined by the researcher.