IGFBP2 Antibody

ProSci
Product Code: PSI-63-534
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-534-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of IGFBP2 Antibody in Jurkat cell line lysates (35ug/lane)(2ug/ml)
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Formalin-fixed and paraffin-embedded human brain tissue with IGFBP2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of jurkat cells using IGFBP2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of IGFBP2 Antibody in Jurkat cell line lysates (35ug/lane)(2ug/ml)
Formalin-fixed and paraffin-embedded human brain tissue with IGFBP2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of jurkat cells using IGFBP2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2, IGFBP2, BP2, IBP2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
IGFBP2-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Background References:
  • Arafat,A.M.,et.al.,J. Clin. Endocrinol. Metab. 94 (12), 5093-5101 (2009)
  • Yazawa,T.,et.al.,Am. J. Pathol. 175 (3), 976-987 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig
Immunogen:
This IGFBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-305 amino acids from the C-terminal region of human IGFBP2.
NCBI Gene ID #:
3485
NCBI Official Name:
Insulin-like growth factor-binding protein 2
NCBI Official Symbol:
IGFBP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
P18065
Protein GI Number:
290457647
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P18065
User NOte:
Optimal dilutions for each application to be determined by the researcher.