LRP12 Antibody

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ProSci
Product Code: PSI-56-027
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-027-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from SW480 cell line,using LRP12 Antibody at 1:1000 at each lane
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LRP12 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of lysate from SW480 cell line,using LRP12 Antibody at 1:1000 at each lane
LRP12 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Low-density lipoprotein receptor-related protein 12, LRP-12, Suppressor of tumorigenicity 7 protein, LRP12, ST7
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the low-density lipoprotein receptor related protein family. The product of this gene is a transmembrane protein that is differentially expressed in many cancer cells. Alternate splicing results in multiple transcript variants.
Background References:
  • Garnis, C., et al. Oncogene 23(14):2582-2586(2004)
  • Battle, M.A., et al. Biochemistry 42(24):7270-7282(2003)
  • Qing, J., et al. Oncogene 18(2):335-342(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse
Immunogen:
This LRP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 635-662 amino acids from the C-terminal region of human LRP12.
NCBI Gene ID #:
29967
NCBI Official Name:
Low-density lipoprotein receptor-related protein 12
NCBI Official Symbol:
LRP12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
95 kDa
Protein Accession #:
Q9Y561
Protein GI Number:
25091287
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q9Y561
User NOte:
Optimal dilutions for each application to be determined by the researcher.