ACTA1/Alpha-actin Antibody
Code | Size | Price |
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PSI-57-981-400ul | 400ul | £662.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
The product encoded by this gene belongs to the actin
family of proteins, which are highly conserved proteins that play a
role in cell motility, structure and integrity. Alpha, beta and
gamma actin isoforms have been identified, with alpha actins being
a major constituent of the contractile apparatus, while beta and
gamma actins are involved in the regulation of cell motility. This
actin is an alpha actin that is found in skeletal muscle. Mutations
in this gene cause nemaline myopathy type 3, congenital myopathy
with excess of thin myofilaments, congenital myopathy with cores,
and congenital myopathy with fiber-type disproportion, diseases
that lead to muscle fiber defects.
Background References:
- Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010) :
- Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010)
- Yu, G., et al.Clin Neurosci 17(6):766-769(2010)
- Yu, C.H., et al. PLoS ONE(7), E11878 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Rabbit, Pig, Chicken, Xenopus, Bovine
Immunogen:
This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the C-terminal region of human ACTA1/Alpha-actin.
NCBI Gene ID #:
58
NCBI Official Name:
Actin, alpha skeletal muscle
NCBI Official Symbol:
ACTA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
P68133
Protein GI Number:
61218043
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P68133
User NOte:
Optimal dilutions for each application to be determined by the researcher.