COL6A1 Antibody

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ProSci
Product Code: PSI-62-346
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-346-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from A431 cell line, using COL6A1 Antibody at 1:1000.
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Western blot analysis of COL6A1 antibody in Y79 cell line lysates (35ug/lane)
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Confocal immunofluorescent analysis of COL6A1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Flow cytometric analysis of 293 cells using COL6A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of lysate from A431 cell line, using COL6A1 Antibody at 1:1000.
Western blot analysis of COL6A1 antibody in Y79 cell line lysates (35ug/lane)
Confocal immunofluorescent analysis of COL6A1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of 293 cells using COL6A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Collagen alpha-1(VI) chain, COL6A1
Application Note:
For WB starting dilution is: 1:500-1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The protein COL6A1 is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.
Background References:
  • Martoni,E., Hum. Mutat. 30 (5), E662-E672 (2009)
  • Kim,T.H., J. Rheumatol. 35 (9), 1849-1852 (2008)
  • Fujita,A., Genet. Mol. Res.(2), 371-378 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This COL6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 831-858 amino acids from the C-terminal region of human COL6A1.
NCBI Gene ID #:
1291
NCBI Official Name:
Collagen alpha-1(VI) chain
NCBI Official Symbol:
COL6A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
109 kDa
Protein Accession #:
P12109
Protein GI Number:
125987811
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
P12109
User NOte:
Optimal dilutions for each application to be determined by the researcher.