VILL Antibody

ProSci
Product Code: PSI-56-221
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-221-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis in K562 cell line lysates (35ug/lane).
2 / 4
VILL Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 4
4 / 4
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in K562 cell line lysates (35ug/lane).
VILL Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Villin-like protein, VILL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene belongs to the villin/gelsolin family. It contains 6 gelsolin-like repeats and a headpiece domain. It may play a role in actin-bundling. [provided by RefSeq].
Background References:
  • Piana, S., et al. J. Mol. Biol. 375(2):460-470(2008)
  • Yamamichi, N., et al. Cancer Res. 67(22):10727-10735(2007)
  • Tomar, A., et al. J. Biol. Chem. 281(42):31972-31986(2006)
  • Ishikawa, S., et al. DNA Res. 4(1):35-43(1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This VILL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-412 amino acids from the Central region of human VILL.
NCBI Gene ID #:
50853
NCBI Official Name:
Villin-like protein
NCBI Official Symbol:
VILL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
96 kDa
Protein Accession #:
O15195
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
O15195
User NOte:
Optimal dilutions for each application to be determined by the researcher.