PKC alpha Antibody
| Code | Size | Price |
|---|
| PSI-62-590-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA
Application Note:
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC alpha is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Background References:
- Rabinovitz, I., et al., Mol. Cell. Biol. 24(10):4351-4360 (2004).
- Gong, N., et al., Proc. Natl. Acad. Sci. U.S.A. 101(17):6490-6495 (2004).
- Hermoso, M., et al., J. Biol. Chem. 279(17):17681-17689 (2004).
- Zemskov, E.A., et al., Neuroreport 14(11):1435-1438 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rabbit, Rat
Immunogen:
This PKC alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-329 amino acids from the C-terminal region of human PKC alpha.
NCBI Gene ID #:
5578
NCBI Official Name:
Protein kinase C alpha type
NCBI Official Symbol:
PRKCA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
P17252
Protein GI Number:
317373571
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
P17252
User NOte:
Optimal dilutions for each application to be determined by the researcher.